Quickstart

This manual covers the Traveller’s assembly, software setup, operation, and maintenance. While the Traveller represents a compact implementation of the openFrame ecosystem, users should note that the framework supports larger and more elaborate designs. These extended systems can include additional imaging modalities and integrations for core imaging facilities. Although beyond the scope of this manual, such expansions remain available for advanced users and institutions seeking enhanced capabilities.

The full assembly guide and user manual is available here: See the Assembly Guide for detailed steps.

1. Place OF01 openFrame on a stable, low‑vibration surface (≥110×80 cm). Do not move after assembly.
2. Mount OF02 Stage & Transillumination: align arrows →1/→2; tighten S02/S03 using 2.5 mm hex (T03).
3. Attach OF03 Transillumination: USB toward pillar; seat onto posts; tighten the two top screws using 2.5 mm hex (T03).
4. Install cameras: OF09 Kikker (mono) and OF11 Rana (color) with labels up; hand‑tighten (do not over tighten).
5. Fit OF04 IRIS‑400: add OF06 ring adaptor (three grub screws 2.0 mm hex (T02)), insert OF07 tube to the guideline, support body on OF08 pedestal ~20 cm left of IRIS port.
6. Cable up:
7. IRIS: OF22 controller OUTPUT ⇄ OF04 via OF19; power via OF23 (arrows up).
8. Cameras: OF16 USB + OF20 12V → Kikker; OF15 USB → Rana.
9. Hub: OF17 → Laptop (P01).
10. Transillumination: OF18 USB‑C → Laptop (P02).
11. Remove transport screws on multiband cube and mirror slider using 3.0 mm hex (T04); store safely.
12. Power laptop; login. Launch Micro‑Manager, choose Default User, config: CellCam MultimodeLED.cfg.
13. Brightfield: filter to Empty, Channel → Mid RGB, Live, exposure 1–50 ms typical.
14. Fluorescence: filter to Multiband, camera Kikker, IRIS R/G/B/UV LED, Live, exposure 100–800 ms typical.