Troubleshooting

Issue	Likely Cause	Resolution
Micro-Manager start-up error	USB devices disconnect or incorrectly connected	Check USB device assignment; restart Micro‑Manager
Image too dark or too bright	Exposure time adjustment	Check histogram and adjust exposure time as required
Image too dark	LED not powered	Check LED connections and power
Poor resolution	Smudged/misaligned lens	Clean objective with blower; refocus