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Histograms 101 (Image Intensity)

  • A histogram shows the distribution of pixel intensities (X = intensity; Y = count).
  • Wide distributions imply higher contrast; narrow imply lower contrast.
  • Avoid clipping at the minimum (0) or maximum (e.g., 4095 for 12‑bit) to preserve detail.

Display Min/Max in Micro‑Manager - In Live view, adjust the histogram sliders to change display range (does not alter underlying pixel values). - Ensure the curve fits within your min/max to avoid hiding real pixels.